The ENO3 Polyclonal Antibody (CAB16371) is a valuable tool for researchers studying enolase 3 (ENO3), an enzyme involved in glycolysis and energy production in cells. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it ideal for use in Western blot applications for the detection and analysis of ENO3 protein levels.ENO3 is crucial for the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, playing a key role in energy metabolism and cellular function. Dysregulation of ENO3 has been implicated in various diseases, including cancer and metabolic disorders.
By targeting ENO3 with this polyclonal antibody, researchers can gain insights into its expression levels and activity in different cell types, advancing studies in fields such as metabolism, oncology, and biochemistry.Overall, the ENO3 Polyclonal Antibody is a valuable tool for investigating the role of ENO3 in cellular metabolism and disease pathology, providing researchers with a reliable means to study this important enzyme in a variety of research applications.
Product Name:
ENO3 Rabbit Polyclonal Antibody
SKU:
CAB16371
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ENO3 (NP_001967.3).
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
2027
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ENO3 Rabbit pAb (CAB16371) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
