The ENO1 Rabbit Monoclonal Antibody is a high-quality research tool designed for the detection and analysis of ENO1, an enzyme involved in glycolysis and also known as alpha-enolase. This antibody, produced using rabbit monoclonal technology, specifically targets the ENO1 protein in human samples and is validated for use in various applications, including Western blotting.ENO1 is a multifunctional protein that plays a key role in energy metabolism and has been implicated in a variety of diseases, including cancer and autoimmune disorders.
Research on ENO1 is important for understanding its role in these conditions and developing targeted therapies. The ENO1 Rabbit Monoclonal Antibody offers researchers a reliable tool for studying ENO1 expression and function in different cell types, providing valuable insights for immunology and cancer research.
Product Name:
ENO1 Rabbit Monoclonal Antibody
SKU:
CAB11448
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ENO1 (P06733).
Cell membrane, Cytoplasm, M line, Nucleus, myofibril, sarcomere.
Calculated MW:
47kDa
Observed MW:
47kDa
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
Purification Method:
Affinity purification
Gene ID:
2023
Clone Number:
ARC0599
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using ENO1 Rabbit mAb (CAB11448) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
