The Alpha-Enolase Polyclonal Antibody (CAB1033) is a valuable tool for researchers studying the Alpha-Enolase protein, which plays a crucial role in glycolysis and is associated with various diseases including cancer and autoimmune disorders. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in applications such as Western blotting and immunohistochemistry.Alpha-enolase is a multifunctional enzyme that not only catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis but also functions as a plasminogen receptor on the cell surface, leading to roles in cell migration and invasion.
Its overexpression has been linked to tumor progression and metastasis in various cancers, making it a promising target for cancer therapy research.By targeting the Alpha-Enolase protein, researchers can gain insights into its role in cancer biology and potentially develop new diagnostic markers or therapeutic strategies. The Alpha-Enolase Polyclonal Antibody (CAB1033) provides a reliable tool for detecting and analyzing Alpha-Enolase in a variety of research settings, making it a valuable asset for studies in oncology and metabolic diseases.
Product Name:
[KO Validated] ENO1 Rabbit Polyclonal Antibody
SKU:
CAB1033
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1 (NP_001419.1).
WB,1:1000 - 1:5000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
NNE; PPH; MPB1; ENO1L1; ENO1-IT1; HEL-S-17;ENO1
Positive Sample:
HeLa,SH-SY5Y,MCF-7,Jurkat,NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm, M line, Nucleus, myofibril, sarcomere.
Calculated MW:
47kDa
Observed MW:
47kDa/
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
Purification Method:
Affinity purification
Gene ID:
2023
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ENO1 Rabbit pAb (CAB1033) at 1:3000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020)Exposure time: 1 s.
