The ENO Polyclonal Antibody (PAC050346) is a valuable tool for researchers studying ENO, a key enzyme in glycolysis and cellular metabolism. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the ENO protein, this antibody facilitates the detection and analysis of ENO levels in various cell types, making it ideal for investigations in metabolic pathways and cancer research.
ENO, also known as enolase, plays a crucial role in catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis. Dysregulation of ENO has been associated with various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. Studying the expression and activity of ENO is essential for understanding its impact on cellular metabolism and potential therapeutic interventions in disease states.
Antibody Name:
eno Antibody (PACO50346)
Antibody SKU:
PACO50346
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:5000
Species Reactivity:
Staphylococcus aureus
Immunogen:
Recombinant Staphylococcus aureus Enolase protein (1-434AA)
Western Blot. Positive WB detected in Recombinant protein. All lanes: eno antibody at 3.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 64 kDa. Observed band size: 64 kDa..
Background:
Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.
Synonyms:
Enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (2-phosphoglycerate dehydratase), eno
UniProt Protein Function:
Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.