The Endog Polyclonal Antibody (CAB0604) is a vital tool for researchers studying endog, a key protein involved in DNA repair processes and apoptosis regulation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various experimental techniques, including Western blotting.Endog, also known as endonuclease G, is a mitochondrial protein that plays an essential role in maintaining genomic stability and cell survival. Its involvement in programmed cell death and DNA fragmentation makes it a crucial target for investigation in the fields of molecular biology and cancer research.
The Endog Polyclonal Antibody enables the detection and analysis of endog protein levels in diverse cell types, offering valuable insights into its function and potential therapeutic applications.The comprehensive understanding of endog function provided by this antibody contributes to the development of innovative strategies for treating diseases characterized by impaired DNA repair mechanisms and dysregulated cell death pathways. Researchers can rely on the Endog Polyclonal Antibody to uncover new insights into the role of endog in health and disease, paving the way for novel therapeutic interventions.
Product Name:
Endo G Rabbit Polyclonal Antibody
SKU:
CAB0604
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 49-297 of human Endo G (NP_004426.2).
The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
Purification Method:
Affinity purification
Gene ID:
2021
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Endo G antibody (CAB0604) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
