The EN2 Polyclonal Antibody (CAB17480) is a valuable tool for research involving EN2, a transcription factor that plays a crucial role in neural development and function. This rabbit polyclonal antibody is highly specific and reactive with human samples, making it ideal for studying EN2 expression and localization in various tissues and cell types.EN2 is involved in the regulation of gene expression during embryonic development, particularly in the formation of the midbrain and cerebellum. Dysregulation of EN2 has been linked to neurodevelopmental disorders such as autism spectrum disorder and schizophrenia, making it a key target for research in neurobiology and neuropsychiatry.
This antibody has been validated for use in Western blot applications, allowing for the detection and analysis of EN2 protein levels in experimental samples. By studying the expression and function of EN2, researchers can gain valuable insights into the molecular mechanisms underlying brain development and disorders, paving the way for potential therapeutic interventions.
Product Name:
EN2 Rabbit Polyclonal Antibody
SKU:
CAB17480
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 250-333 of human EN2 (NP_001418.2).
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
Purification Method:
Affinity purification
Gene ID:
2020
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EN2 Rabbit pAb (CAB17480) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
