The EML4 Polyclonal Antibody (CAB16117) is a valuable tool for researchers studying the EML4 gene, which is known to be involved in the formation of fusion genes in various cancers, particularly non-small cell lung cancer. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.EML4, or echinoderm microtubule-associated protein-like 4, is a key player in the development and progression of certain cancer types. Its fusion with other genes can lead to the activation of oncogenic pathways, making it a promising target for therapeutic interventions.
By detecting and analyzing EML4 protein levels in different cell types, researchers can gain insights into the mechanisms underlying cancer progression and potentially identify new therapeutic strategies.The EML4 Polyclonal Antibody is an essential tool for studies in oncology and molecular biology, providing researchers with the means to investigate the role of EML4 in cancer development and progression. Its high specificity and reactivity make it a reliable choice for experiments aimed at understanding the molecular mechanisms of EML4-driven oncogenesis.
Product Name:
EML4 Rabbit Polyclonal Antibody
SKU:
CAB16117
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 360-560 of human EML4 (NP_061936.2).
This gene is a member of the echinoderm microtubule associated protein-like family. The encoded WD-repeat protein may be involved in microtubule formation. Abnormal fusion of parts of this gene with portions of the anaplastic lymphoma receptor tyrosine kinase gene, which generates EML4-ALK fusion transcripts, is one of the primary mutations associated with non-small cell lung cancer. Alternative splicing of this gene results in two transcript variants.
Purification Method:
Affinity purification
Gene ID:
27436
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using EML4 Rabbit pAb (CAB16117) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
