The EME1 Polyclonal Antibody (CAB8375) is a valuable tool for researchers studying EME1, a key protein involved in DNA repair processes. This antibody, produced in rabbit hosts, has high specificity for EME1 and has been validated for use in Western blotting applications. By binding to the EME1 protein, researchers can accurately detect and analyze levels of EME1 in various cell types, making it an essential reagent for studies in molecular biology and cancer research.EME1 is a critical component of the DNA repair machinery, playing a crucial role in maintaining genomic stability and preventing the accumulation of DNA damage that can lead to cancer and other diseases.
Understanding the function of EME1 is essential for developing targeted therapies that can enhance DNA repair mechanisms in cancer cells or sensitize them to DNA-damaging treatments. With its high specificity and reliability, the EME1 Polyclonal Antibody is an indispensable tool for unraveling the complex mechanisms of DNA repair and exploring new therapeutic strategies.
Product Name:
EME1 Rabbit Polyclonal Antibody
SKU:
CAB8375
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human EME1 (NP_689676.2).
This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
146956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EME1 antibody (CAB8375) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
