The EMCN Polyclonal Antibody (CAB14131) is a valuable tool for researchers studying EMCN, a glycoprotein highly expressed on the surface of endothelial cells in human tissues. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in various applications, including Western blot analysis. By specifically binding to the EMCN protein, this antibody enables precise detection and analysis in different cell types, making it ideal for investigations in vascular biology, inflammation, and cancer research.EMCN, also known as endomucin, plays a crucial role in regulating endothelial cell function, vascular development, and angiogenesis.
Its involvement in cell adhesion and blood vessel formation underscores its importance in various physiological and pathological processes. Research on EMCN is key to understanding vascular biology and developing targeted therapies for diseases such as cardiovascular disorders and cancer.In summary, the EMCN Polyclonal Antibody (CAB14131) offers researchers a reliable tool for studying the role of EMCN in endothelial cell biology and related diseases, providing valuable insights for potential therapeutic interventions.
Antibody Name:
Anti-EMCN Antibody
Antibody SKU:
CAB14131
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IHC
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-135 of human EMCN (NP_057326.2).
Application:
WB IHC
Recommended Dilution:
WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:
Human, Mouse, Rat
Positive Samples:
A-549, SGC-7901, Mouse brain, Rat spinal cord
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-135 of human EMCN (NP_057326.2).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cell membrane, Membrane, Secreted, Single-pass type I membrane protein, Single-pass type I membrane protein
Calculated MW:
18kDa/26kDa/27kDa
Observed MW:
36kDa
Synonyms:
EMCN, EMCN2, MUC14, endomucin
Background:
UniProt Protein Function:
EMCN: Endothelial sialomucin, also called endomucin or mucin- like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: 4q24
Cellular Component: integral to membrane; plasma membrane; extracellular region
NCBI Summary:
EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM, Mar 2008]
endomucin; MUC-14; mucin-14; endomucin-2; gastric cancer antigen Ga34
UniProt Protein Name:
Endomucin
UniProt Synonym Protein Names:
Endomucin-2; Gastric cancer antigen Ga34; Mucin-14; MUC-14
Protein Family:
Endomucin
UniProt Gene Name:
EMCNÂ Â
UniProt Entry Name:
MUCEN_HUMAN
Western blot analysis of extracts of various cell lines, using EMCN antibody (CAB14131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
