The ELOVL6 Polyclonal Antibody (CAB21094) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, exhibits high specificity against human samples and has been validated for use in Western blot applications. By targeting the ELOVL6 protein, this antibody enables precise detection and analysis in various cell types, making it an indispensable asset for studies in metabolic disorders, lipid metabolism, and obesity-related research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ELOVL6 Polyclonal Antibody
SKU:
CAB21094
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FAE, LCE, FACE, hELO2, ELOVL6
Positive Sample:
U-251MG, HepG2, SK-BR-3, Mouse liver, Rat liver, Mouse kidney, Rat liver, Mouse kidney
Cellular Localization:
Endoplasmic Reticulum.
Calculated MW:
31kDa
Observed MW:
31kDa
Fatty acid elongases (EC 6.2.1.3), such as ELOVL6, use malonyl-CoA as a 2-carbon donor in the first and rate-limiting step of fatty acid elongation (Moon et al., 2001 [PubMed 11567032]).
Purification Method
Affinity purification
Gene ID
79071
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse kidney using ELOVL6 Rabbit pAb (CAB21094) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time:30s.
Western blot analysis of lysates from Rat liver using ELOVL6 Rabbit pAb (CAB21094) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020) Exposure time: 10 s.
