The Elane Polyclonal Antibody (CAB13015) is a vital tool for researchers studying Elane, a protease enzyme found in neutrophil granules that plays a crucial role in the immune system's response to infections and inflammation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.Elane is involved in the process of neutrophil extracellular trap (NET) formation, where neutrophils release their DNA to trap and kill pathogens. Dysregulation of Elane activity has been linked to various inflammatory conditions, making it a promising target for research in immunology, infectious diseases, and autoimmune disorders.
This antibody specifically binds to Elane protein, allowing for its detection and analysis in different cell types, providing valuable insights into its functions and potential therapeutic applications.Understanding the role of Elane in immune responses and inflammatory pathways is essential for developing targeted therapies and interventions for a range of diseases and conditions. The Elane Polyclonal Antibody is a reliable tool for investigating the mechanisms underlying Elane's function, paving the way for novel discoveries and advancements in the field of immunology and disease research.
Product Name:
ELANE Rabbit Polyclonal Antibody
SKU:
CAB13015
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 30-267 of human Neutrophil Elastase (ELANE) (NP_001963.1).
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.
Purification Method:
Affinity purification
Gene ID:
1991
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from THP-1 cells, using Neutrophil Elastase (ELANE) Rabbit pAb (CAB13015) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
