The EIF5B Polyclonal Antibody (CAB15123) is a valuable tool for researchers studying EIF5B, a key protein involved in the initiation of protein synthesis. This antibody, produced in rabbits, displays high specificity and sensitivity towards human samples, making it a reliable choice for Western blot applications. By binding to EIF5B, it enables researchers to detect and analyze the protein in various cell types, facilitating studies in molecular biology and cancer research.EIF5B is a critical component of the translation initiation complex, playing a crucial role in the accurate start of protein synthesis. Dysregulation of EIF5B has been linked to various diseases, including cancer, making it an intriguing target for therapeutic intervention.
By gaining insights into the function and regulation of EIF5B, researchers can pave the way for the development of novel treatments for EIF5B-associated disorders.Overall, the EIF5B Polyclonal Antibody is a reliable tool for scientists investigating the role of EIF5B in protein synthesis and disease development. Its high specificity and sensitivity make it an essential reagent for studies aiming to unravel the intricate mechanisms of translation initiation and its implications in health and disease.
Product Name:
EIF5B Rabbit Polyclonal Antibody
SKU:
CAB15123
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human EIF5B (NP_056988.3).
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.
Purification Method:
Affinity purification
Gene ID:
9669
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EIF5B Rabbit pAb (CAB15123).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
