EIF5B Rabbit Polyclonal Antibody (CAB15123)
- SKU:
- CAB15123
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
Product Name: | EIF5B Rabbit Polyclonal Antibody |
SKU: | CAB15123 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human EIF5B (NP_056988.3). |
Sequence: | MGKK QKNK SEDS TKDD IDLD ALAA EIEG AGAA KEQE PQKS KGKK KKEK KKQD FDED DILK ELEE LSLE AQGI KADR ETVA VKPT ENNE EEFT SKDK KKKG QKGK KQSF DDND SEEL EDKD SKSK KTAK PKVE MYSG SDDD DDFN KLPK KAKG KAQK SNKK WDGS EEDE DNSK KIKE RSRI NSSG ESGD ESDE FLQS RKGQ KKNQ KNKP GPNI ESGN EDDD ASFK IKTV AQKK AEKK ERER KKRD EEKA KLRK LKEK EELE TGKK DQSK QKES Q |
Tested Applications: | WB IF/ICC ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 |
Synonyms: | IF2; EIF5B |
Positive Sample: | DU145,HeLa,Jurkat,LO2 |
Conjugate: | Unconjugated |
Cellular Localization: | Cytoplasm. |
Calculated MW: | 139kDa |
Observed MW: | 179kDa |
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.
Purification Method: | Affinity purification |
Gene ID: | 9669 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |