The EIF5 Polyclonal Antibody (CAB6583) is a valuable tool for researchers studying eukaryotic translation initiation factor 5 (EIF5), a key player in protein synthesis. This antibody, produced in rabbits, is highly specific for EIF5 and has been validated for use in Western blot applications with human samples.EIF5 is involved in the assembly of the ribosomal preinitiation complex and plays a crucial role in the regulation of translation initiation. Dysregulation of EIF5 has been linked to various diseases, including cancer and neurodegenerative disorders, making it an important target for research in molecular biology and translational medicine.
By using the EIF5 Polyclonal Antibody, researchers can accurately detect and analyze EIF5 protein levels in different cell types, providing valuable insights into its function and potential therapeutic applications. This antibody is a reliable tool for investigating the role of EIF5 in various cellular processes and diseases, advancing our understanding of protein synthesis mechanisms and opening up new avenues for targeted treatment strategies.
Product Name:
EIF5 Rabbit Polyclonal Antibody
SKU:
CAB6583
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-235 of human EIF5 (NP_892116.2).
Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).
Purification Method:
Affinity purification
Gene ID:
1983
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EIF5 antibody (CAB6583) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
