The EIF4G3 Polyclonal Antibody (CAB14621) is a valuable tool for researchers studying EIF4G3, a key protein involved in the initiation of translation. This antibody, produced in rabbits, is highly specific to human samples and has been verified for use in Western blotting applications. By binding to EIF4G3, this antibody allows for the accurate detection and analysis of this protein in a variety of cell types, making it an essential component of studies in the fields of molecular biology and genetics.EIF4G3 is a critical factor in the regulation of protein synthesis, playing a pivotal role in the assembly of the eukaryotic translation initiation complex.
Its function in the control of gene expression makes it a target of interest in research on a wide range of biological processes, including cell growth, development, and disease. By studying EIF4G3, researchers can gain valuable insights into the mechanisms underlying these processes and potentially uncover new therapeutic targets for conditions such as cancer and metabolic disorders.
Product Name:
EIF4G3 Rabbit Polyclonal Antibody
SKU:
CAB14621
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1200 of human EIF4G3 (NP_003751.2).
The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p.
Purification Method:
Affinity purification
Gene ID:
8672
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EIF4G3 Rabbit pAb (CAB14621) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
