The EIF4G2 Polyclonal Antibody (CAB2897) is a valuable tool for researchers studying EIF4G2, a protein involved in translation initiation and mRNA binding. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically targeting the EIF4G2 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it essential for studies in molecular biology and cancer research.EIF4G2, also known as eukaryotic translation initiation factor 4 gamma 2, plays a crucial role in protein synthesis regulation and has been implicated in various diseases, including cancer.
Understanding the function and regulation of EIF4G2 is key to developing targeted therapies for conditions characterized by dysregulated protein synthesis. The EIF4G2 Polyclonal Antibody provides researchers with a powerful tool to investigate the role of EIF4G2 in cellular processes and disease progression.
Product Name:
EIF4G2 Rabbit Polyclonal Antibody
SKU:
CAB2897
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 320-490 of human EIF4G2/p97 (NP_001409.3).
Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
Purification Method:
Affinity purification
Gene ID:
1982
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EIF4G2/p97 Rabbit pAb (CAB2897) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
