EIF4G1 Rabbit Polyclonal Antibody (CAB7552)
- SKU:
- CAB7552
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
Product Name: | EIF4G1 Rabbit Polyclonal Antibody |
SKU: | CAB7552 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3). |
Sequence: | PEEL LNGA PSPP AVDL SPVS EPEE QAKE VTAS MAPP TIPS ATPA TAPS ATSP AQEE EMEE EEEE EEGE AGEA GEAE SEKG GEEL LPPE STPI PANL SQNL E |
Tested Applications: | WB IHC-P IP ELISA |
Recommended Dilution: | WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
Synonyms: | P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1; EIF4G1 |
Positive Sample: | HeLa |
Conjugate: | Unconjugated |
Cellular Localization: | cytoplasm, cytoplasmic stress granule, cytosol, eukaryotic translation initiation factor 4F complex, nucleus, postsynapse |
Calculated MW: | 175kDa |
Observed MW: | 220kDa |
The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
Purification Method: | Affinity purification |
Gene ID: | 1981 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |