The eIF4G1 Polyclonal Antibody (CAB0881) is a valuable tool for researchers studying eIF4G1, a key protein involved in translation initiation. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to eIF4G1, researchers can accurately detect and analyze this protein in various cell types, making it an essential asset for studies in molecular biology and cancer research.eIF4G1 is a critical component of the eIF4F complex, which plays a crucial role in the initiation of protein synthesis. Dysregulation of eIF4G1 has been linked to various diseases, including cancer, making it a promising target for therapeutic interventions.
Understanding the function and regulation of eIF4G1 is essential for developing targeted therapies that can potentially inhibit the growth and progression of cancer cells.Overall, the eIF4G1 Polyclonal Antibody (CAB0881) serves as a valuable tool for researchers looking to delve deeper into the role of eIF4G1 in cellular processes and disease states, ultimately paving the way for new advancements in the field of molecular biology and cancer research.
Product Name:
EIF4G1 Rabbit Polyclonal Antibody
SKU:
CAB0881
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human EIF4G1 (NP_886553.3).
Sequence:
AGSN PGPE SEGS GVPP RPEE ADET WDSK EDKI HNAE NIQP GEQK YEYK SDQW KPLN LEEK KRYD REFL LGFQ FIFA SMQK PEGL PHIS DVVL DKAN KTPL R
The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
Purification Method:
Affinity purification
Gene ID:
1981
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of HeLa, using EIF4G1 Rabbit pAb (CAB0881) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
