The eIF4EBP1 Polyclonal Antibody is specifically designed for research involving eIF4EBP1, a key protein involved in the regulation of protein synthesis. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the eIF4EBP1 protein, this antibody enables the detection and analysis of this critical protein in various cell types, making it an essential tool for studies in molecular biology and cancer research.eIF4EBP1, also known as eukaryotic initiation factor 4E-binding protein 1, plays a crucial role in the control of translation initiation, a process essential for protein synthesis.
Dysregulation of eIF4EBP1 has been linked to various diseases, including cancer and neurological disorders, making it a promising target for therapeutic interventions. Understanding the function of eIF4EBP1 is vital for the development of strategies aimed at modulating protein synthesis in disease states.Overall, the eIF4EBP1 Polyclonal Antibody serves as a valuable tool for researchers interested in studying the role of eIF4EBP1 in cellular processes and disease development. Its high reactivity, specificity, and reliability make it a preferred choice for experiments aiming to elucidate the intricate mechanisms of protein synthesis regulation.
Product Name:
eIF4EBP1 Polyclonal Antibody
SKU:
CAB21386
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 50-118 of human eIF4EBP1. (NP_004086.1).
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Purification Method:
Affinity purification
Gene ID:
1978
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KO Validated] eIF4EBP1 Rabbit pAb (CAB21386) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
