The EIF4E Rabbit Monoclonal Antibody (CAB19044) is a valuable tool for researchers studying EIF4E, a key protein involved in the regulation of mRNA translation. This antibody, produced in rabbits, exhibits high specificity and sensitivity for EIF4E in a variety of sample types, particularly human tissues. It has been validated for use in Western blotting, immunohistochemistry, and immunofluorescence applications, allowing for precise detection and analysis of EIF4E expression.EIF4E is a critical component of the eukaryotic translation initiation complex and is essential for the synthesis of proteins involved in cell growth, proliferation, and differentiation.
Dysregulation of EIF4E has been implicated in various diseases, including cancer, neurological disorders, and developmental disorders. By studying EIF4E expression and activity using this antibody, researchers can gain insights into the molecular mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention.
Product Name:
eIF4E Rabbit Monoclonal Antibody
SKU:
CAB19044
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 118-217 of human eIF4E (P06730).
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1977
Clone Number:
ARC0447
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using eIF4E Rabbit mAb (CAB19044) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
