The eIF4E Polyclonal Antibody (CAB6085) is an essential tool for researchers studying eIF4E, a key player in the regulation of protein synthesis and mRNA translation. This polyclonal antibody is raised in rabbits and exhibits high reactivity with human samples, making it an ideal choice for Western blot applications.eIF4E, also known as eukaryotic translation initiation factor 4E, plays a crucial role in controlling the translation of specific mRNAs involved in cell growth, proliferation, and differentiation. Dysregulation of eIF4E has been linked to a variety of diseases, including cancer, making it an attractive target for therapeutic intervention.
By targeting the eIF4E protein, researchers can gain insights into the complex mechanisms of protein synthesis and its implications for various diseases. The eIF4E Polyclonal Antibody (CAB6085) enables the detection and analysis of eIF4E in a wide range of cell types, supporting studies in cancer research, molecular biology, and drug development. Stay at the forefront of cutting-edge research with this versatile and reliable antibody.
Product Name:
eIF4E Rabbit Polyclonal Antibody
SKU:
CAB6085
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4E (NP_001959.1).
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1977
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using eIF4E antibody (CAB6085) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.