The EIF4E Polyclonal Antibody (CAB2162) is a valuable tool for researchers studying EIF4E, a protein involved in the regulation of protein synthesis and cell growth. This antibody is derived from rabbits and is highly sensitive to human samples, making it an ideal choice for Western blot applications.EIF4E is a key player in the initiation of translation, a process critical for protein production in cells. Dysregulation of EIF4E has been linked to various diseases, including cancer, making it a promising target for therapeutic interventions.
By specifically binding to EIF4E, this antibody allows for precise detection and analysis of the protein in different cell types, facilitating studies in molecular biology and cancer research.Understanding the role of EIF4E in cellular processes is essential for uncovering its implications in disease development and progression. By utilizing the EIF4E Polyclonal Antibody, researchers can further explore the functions and mechanisms of EIF4E, paving the way for the development of novel therapeutic strategies targeting this protein.
Product Name:
eIF4E Rabbit Polyclonal Antibody
SKU:
CAB2162
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4E (NP_001959.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:100 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1; 4E
Positive Sample:
293T,HeLa,K-562
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, P-body.
Calculated MW:
25kDa
Observed MW:
25kDa/28kDa
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1977
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KD Validated] eIF4E Rabbit pAb (CAB2162) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 45s.
