The eIF4E Polyclonal Antibody (CAB0468) is a valuable tool for research involving eIF4E, a key protein involved in regulating protein synthesis and cell growth. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications. By targeting the eIF4E protein, this antibody enables researchers to study its function and activity in various cell types, providing insights into its role in cancer development, viral infection, and other cellular processes.
eIF4E, also known as eukaryotic translation initiation factor 4E, is a critical player in the initiation of translation of mRNA into proteins. Dysregulation of eIF4E has been implicated in a variety of diseases, including cancer, neurological disorders, and viral infections, making it an attractive target for therapeutic interventions. By utilizing the eIF4E Polyclonal Antibody, researchers can explore the molecular mechanisms underlying these diseases and potentially identify new strategies for treatment and intervention.
Product Name:
eIF4E Rabbit Polyclonal Antibody
SKU:
CAB0468
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human eIF4E (NP_001959.1).
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1977
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using eIF4E antibody (CAB0468) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
