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Anti-EIF4B Antibody (CAB5405)

SKU:
CAB5405
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism
  • Metabolism Antibodies 2 Anti-EIF4B Antibody CAB5405
  • Metabolism Antibodies 2 Anti-EIF4B Antibody CAB5405
  • Metabolism Antibodies 2 Anti-EIF4B Antibody CAB5405
  • Metabolism Antibodies 2 Anti-EIF4B Antibody CAB5405
  • Metabolism Antibodies 2 Anti-EIF4B Antibody CAB5405
€129 - €359
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Description

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Antibody Name:Anti-EIF4B Antibody
Antibody SKU:CAB5405
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:A-549, HeLa, 293T, MCF7, 22Rv1
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SQST RAAS IFGG AKPV DTAA RERE VEER LQKE QEKL QRQL DEPK LERR PRER HPSW RSEE TQER ERSR TGSE SSQT GTST TSSR NARR RESE KSLE NETL NKEE DCHS PTSK PPKP DQPL KVMP APPP KENA WVKR SSNP PARS QSSD TEQQ SPTS GGGK VAPA QPSE EGPG RKDE NKVD GMNA PKGQ TGNS SRGP GDGG NRDH WKES DRKD GKKD QDSR SAPE PKKP EENP ASKF SSAS KYAA LSVD GEDE NEGE DYAE
Gene ID:1975
Uniprot:P23588
Cellular Location:
Calculated MW:64kDa/69kDa
Observed MW:80kDa
Synonyms:EIF4B, EIF-4B, PRO1843
Background:
UniProt Protein Function:EIF4B: eukaryotic translation initiation factor 4B. Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
UniProt Protein Details:

Protein type:RNA-binding; Translation initiation; Translation

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytosol; eukaryotic translation initiation factor 4F complex; polysome

Molecular Function:helicase activity; protein binding; ribosomal small subunit binding; RNA binding; RNA strand annealing activity; RNA strand-exchange activity

Biological Process: formation of translation preinitiation complex; poly(A) tail shortening; regulation of translational initiation; translational initiation

UniProt Code:P23588
NCBI GenInfo Identifier:205371761
NCBI Gene ID:1975
NCBI Accession:P23588.2
UniProt Secondary Accession:P23588,Q4G0E3, Q53HQ2, Q6GPH5, Q6IB46, Q8WYK5, B4DS13
UniProt Related Accession:P23588
Molecular Weight:64,805 Da
NCBI Full Name:Eukaryotic translation initiation factor 4B
NCBI Synonym Full Names:eukaryotic translation initiation factor 4B
NCBI Official Symbol:EIF4B  
NCBI Official Synonym Symbols:EIF-4B; PRO1843  
NCBI Protein Information:eukaryotic translation initiation factor 4B
UniProt Protein Name:Eukaryotic translation initiation factor 4B
Protein Family:Eukaryotic translation initiation factor
UniProt Gene Name:EIF4B  
UniProt Entry Name:IF4B_HUMAN
Anti-EIF4B Antibody (CAB5405)Western blot analysis of extracts of various cell lines, using EIF4B antibody (CAB5405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-EIF4B Antibody (CAB5405)Immunohistochemistry of paraffin-embedded human liver cancer using EIF4B Antibody (CAB5405) at dilution of 1:100 (40x lens).
Anti-EIF4B Antibody (CAB5405)Immunohistochemistry of paraffin-embedded human prostate using EIF4B Antibody (CAB5405) at dilution of 1:100 (40x lens).
Anti-EIF4B Antibody (CAB5405)Immunohistochemistry of paraffin-embedded human esophagus using EIF4B Antibody (CAB5405) at dilution of 1:100 (40x lens).
Anti-EIF4B Antibody (CAB5405)Immunofluorescence analysis of A549 cells using EIF4B antibody (CAB5405). Blue: DAPI for nuclear staining.

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