The EIF4A2 Polyclonal Antibody (CAB3849) is a valuable research tool designed for studying EIF4A2, a key protein involved in RNA metabolism and translation initiation. This antibody, raised in rabbits, is highly specific and reacts with human samples, making it suitable for various research applications.EIF4A2 is a member of the EIF4A family of RNA helicases, playing a crucial role in unwinding RNA secondary structures during translation initiation. Dysregulation of EIF4A2 has been associated with various diseases, including cancer, making it a promising target for therapeutic intervention.
The EIF4A2 Polyclonal Antibody enables researchers to detect and analyze EIF4A2 expression in different cell types, aiding in the understanding of its function and dysregulation in disease states. This antibody is validated for use in techniques such as Western blotting, immunofluorescence, and immunoprecipitation, providing researchers with a versatile tool for their studies in molecular biology and cancer research.
Product Name:
EIF4A2 Rabbit Polyclonal Antibody
SKU:
CAB3849
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF4A2 (NP_001958.2).
cytosol, eukaryotic translation initiation factor 4F complex, perinuclear region of cytoplasm
Calculated MW:
46kDa
Observed MW:
46kDa
Enables ATP hydrolysis activity. Involved in negative regulation of RNA-directed 5'-3' RNA polymerase activity. Located in perinuclear region of cytoplasm.
Purification Method:
Affinity purification
Gene ID:
1974
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EIF4A2 antibody (CAB3849) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
