The EIF3K Polyclonal Antibody (CAB9969) is a valuable tool for researchers studying EIF3K, a subunit of the eukaryotic translation initiation factor 3 (eIF3) complex. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it suitable for Western blot applications. By targeting the EIF3K protein, this antibody enables the detection and analysis of EIF3K expression in various cell types, providing insights into translation initiation processes and potential therapeutic targets.EIF3K is a crucial component of the eIF3 complex, which plays a central role in protein synthesis by facilitating ribosome recruitment to mRNA.
Dysregulation of EIF3K has been implicated in various diseases, including cancer and neurological disorders, highlighting its importance in cellular processes. By investigating EIF3K using this polyclonal antibody, researchers can gain a better understanding of its function and potential implications in disease pathology, paving the way for targeted therapeutic interventions and advancements in translational research.
Product Name:
EIF3K Rabbit Polyclonal Antibody
SKU:
CAB9969
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-218 of human EIF3K (NP_037366.1).
The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).
Purification Method:
Affinity purification
Gene ID:
27335
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EIF3K antibody (CAB9969) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
