The EIF3F Polyclonal Antibody (CAB7023) is an essential tool for research involving EIF3F, a key regulatory subunit of the eukaryotic translation initiation factor 3 (eIF3) complex. This antibody, produced in rabbits, has high specificity and sensitivity for detecting EIF3F in human samples, making it ideal for use in Western blot applications.EIF3F plays a crucial role in the initiation of protein synthesis, making it a vital component in cellular processes such as cell growth and proliferation. Dysfunction of EIF3F has been implicated in various diseases, including cancer, making it an important target for research in oncology and molecular biology.
By using the EIF3F Polyclonal Antibody, researchers can accurately detect and analyze EIF3F expression in different cell types, providing valuable insights into its function and potential role in disease development. This antibody is a valuable tool for studies aimed at understanding the molecular mechanisms underlying EIF3F activity and its impact on cellular processes, offering new opportunities for therapeutic intervention in cancer and other diseases.
Product Name:
EIF3F Rabbit Polyclonal Antibody
SKU:
CAB7023
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 88-357 of human EIF3F (NP_003745.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
MRT67; EIF3S5; eIF3-p47; EIF3F
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
38kDa
Observed MW:
38kDa
Enables deubiquitinase activity and identical protein binding activity. Contributes to translation initiation factor activity. Involved in IRES-dependent viral translational initiation; protein deubiquitination; and translational initiation. Located in membrane. Part of eukaryotic translation initiation factor 3 complex. Implicated in autosomal recessive non-syndromic intellectual disability.
Purification Method:
Affinity purification
Gene ID:
8665
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using EIF3F Rabbit pAb (CAB7023) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
