The EIF3E Polyclonal Antibody (CAB5447) is a valuable tool for researchers studying EIF3E, a subunit of the eukaryotic translation initiation factor 3 (eIF3) complex involved in protein synthesis regulation. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it suitable for Western blot applications. By targeting the EIF3E protein, researchers can effectively investigate its role in protein translation and its potential implications in cellular processes.eIF3E is a crucial component of the eIF3 complex, which plays a fundamental role in the initiation of protein synthesis.
Dysregulation of eIF3E has been linked to various diseases, including cancer and neurodegenerative disorders, highlighting its importance in cellular homeostasis. The EIF3E Polyclonal Antibody enables researchers to explore the functions and mechanisms of EIF3E, providing insights that can contribute to the development of targeted therapies for conditions associated with aberrant protein synthesis.
Product Name:
eIF3e Rabbit Polyclonal Antibody
SKU:
CAB5447
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-445 of human eIF3e (NP_001559.1).
Enables protein N-terminus binding activity. Contributes to translation initiation factor activity. Involved in positive regulation of mRNA binding activity; regulation of gene expression; and translational initiation. Located in cytosol and nucleus. Part of eukaryotic translation initiation factor 3 complex. Colocalizes with PML body.
Purification Method:
Affinity purification
Gene ID:
3646
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using eIF3e antibody (CAB5447) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
