Anti-EIF2AK4 Antibody (CAB7155)

Antibody Name:	Anti-EIF2AK4 Antibody
Antibody SKU:	CAB7155
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EIF2AK4 (NP_001013725.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EIF2AK4 (NP_001013725.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGG RGAP GRGR DEPP ESYP QRQD HELQ ALEA IYGA DFQD LRPD ACGP VKEP PEIN LVLY PQGL TGEE VYVK VDLR VKCP PTYP DVVP EIEL KNAK GLSN ESVN LLKS RLEE LAKK HCGE VMIF ELAY HVQS FLSE HNKP PPKS FHEE MLER RAQE EQQR LLEA KRKE EQEQ REIL HEIQ RRKE EIKE EKKR KEMA KQER LEIA SLSN QDHT SKKD PGGH RTAA ILHG GSPD FVGN GKHR ANSS GRSR RERQ YSVC NSED SPGS CEIL YFNM GSPD QLMV HKGK CIGS DEQL GKLV YNAL
Gene ID:	440275
Uniprot:	Q9P2K8
Cellular Location:
Calculated MW:	69kDa/183kDa/186kDa
Observed MW:	220kDa

Synonyms:

EIF2AK4, GCN2, PVOD2

Background:

This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.

UniProt Protein Function:	GCN2: a kinase of the PEK family. Phosphorylates and inhibits eukaryotic initiation factor (eIF)-2 alpha, providing an important protective mechanism during various cellular stress conditions including endoplasmic reticulum (ER) stress.
UniProt Protein Details:	Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Translation; Other group; PEK family; GCN2 subfamily Chromosomal Location of Human Ortholog: 15q15.1 Molecular Function:protein serine/threonine kinase activity; eukaryotic translation initiation factor 2alpha kinase activity; ATP binding Biological Process: unfolded protein response; negative regulation of translation; cellular response to starvation; protein amino acid phosphorylation; regulation of translational initiation Disease: Pulmonary Venoocclusive Disease 2, Autosomal Recessive
NCBI Summary:	This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014]
UniProt Code:	Q9P2K8
NCBI GenInfo Identifier:	296439368
NCBI Gene ID:	440275
NCBI Accession:	Q9P2K8.3
UniProt Secondary Accession:	Q9P2K8,Q69YL7, Q6DC97, Q96GN6, Q9H5K1, Q9NSQ3, Q9NSZ5 Q9UJ56, C9JEC4,
UniProt Related Accession:	Q9P2K8
Molecular Weight:
NCBI Full Name:	Eukaryotic translation initiation factor 2-alpha kinase 4
NCBI Synonym Full Names:	eukaryotic translation initiation factor 2 alpha kinase 4
NCBI Official Symbol:	EIF2AK4
NCBI Official Synonym Symbols:	GCN2; PVOD2
NCBI Protein Information:	eukaryotic translation initiation factor 2-alpha kinase 4; GCN2-like protein; GCN2 eIF2alpha kinase; general control nonderepressible 2
UniProt Protein Name:	Eukaryotic translation initiation factor 2-alpha kinase 4
UniProt Synonym Protein Names:	GCN2-like protein
UniProt Gene Name:	EIF2AK4
UniProt Entry Name:	E2AK4_HUMAN

	Western blot analysis of extracts of HepG2 cells, using EIF2AK4 antibody (CAB7155) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using EIF2AK4 antibody (CAB7155) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using EIF2AK4 antibody (CAB7155) at dilution of 1:100 (40x lens).

Antibodies

Anti-EIF2AK4 Antibody (CAB12618)

Anti-GCN2 Antibody (CAB2307)

Phospho-EIF2AK4 (T899) Antibody (PACO04351)

EIF2AK4 Antibody (PACO04352)

EIF2AK4 Antibody (PACO09034)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)