The EIF2AK4 Polyclonal Antibody (CAB12618) is a valuable tool for researchers studying EIF2AK4, a key enzyme involved in cellular stress responses and protein synthesis regulation. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it an ideal choice for Western blot applications.EIF2AK4, also known as general control nonderepressible 2 (GCN2) protein kinase, plays a crucial role in the integrated stress response pathway by phosphorylating eukaryotic translation initiation factor 2 alpha (eIF2α) in response to amino acid deprivation and other stress signals.
Dysregulation of EIF2AK4 has been linked to various diseases, including cancer and metabolic disorders, highlighting its importance in cellular homeostasis.By using the EIF2AK4 Polyclonal Antibody, researchers can effectively detect and study EIF2AK4 levels and activity in different cell types, paving the way for a deeper understanding of its function in disease pathogenesis. This antibody is a valuable tool for investigations in cell biology, molecular biology, and potentially for the development of targeted therapies in the future.
Product Name:
GCN2 Rabbit Polyclonal Antibody
SKU:
CAB12618
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCN2 (NP_001013725.2).
This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.
Purification Method:
Affinity purification
Gene ID:
440275
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using GCN2 Rabbit pAb (CAB12618) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
