The EIF2AK3 Polyclonal Antibody is a valuable tool for research involving EIF2AK3, a key regulator of cellular stress and unfolded protein response pathways. This rabbit polyclonal antibody is highly specific for human samples and has been validated for use in Western blot applications. By binding to the EIF2AK3 protein, this antibody enables the detection and analysis of this important molecule in various cell types, making it ideal for studies in cell biology, immunology, and disease research.EIF2AK3, also known as PERK, is a critical mediator of the unfolded protein response, playing a key role in the cellular adaptation to stress conditions such as endoplasmic reticulum stress.
Dysregulation of EIF2AK3 activity has been implicated in various diseases, including neurodegenerative disorders, diabetes, and cancer. Research into EIF2AK3 function is essential for understanding its role in these diseases and developing targeted therapies to modulate its activity.Overall, the EIF2AK3 Polyclonal Antibody is a valuable tool for researchers studying cellular stress responses, protein folding pathways, and disease mechanisms. Its high specificity and sensitivity make it a reliable reagent for Western blot analysis, enabling accurate and reproducible data in a variety of experimental settings.
Product Name:
EIF2AK3 Rabbit Polyclonal Antibody
SKU:
CAB18196
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 867-1116 of human PERK (NP_004827.4).
Endoplasmic reticulum membrane, Single-pass type I membrane protein.
Calculated MW:
125kDa
Observed MW:
140kDa
The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome.
Purification Method:
Affinity purification
Gene ID:
9451
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse thymus, using PERK Rabbit pAb (CAB18196) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
