The EIF2AK1 Polyclonal Antibody (CAB3415) is a crucial tool for researchers studying EIF2AK1, a key protein involved in the regulation of translation initiation in response to cellular stress. This antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding specifically to the EIF2AK1 protein, this antibody enables accurate detection and analysis in various cell types, making it an essential asset for studies in molecular biology and cancer research.EIF2AK1, also known as PKR-like endoplasmic reticulum kinase (PERK), plays a pivotal role in the integrated stress response pathway, which is essential for maintaining cellular homeostasis under stressful conditions such as nutrient deprivation or accumulation of misfolded proteins.
Dysregulation of EIF2AK1 activity has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. Therefore, understanding the molecular mechanisms and functions of EIF2AK1 is crucial for developing targeted therapies and diagnostic tools for these conditions.
Product Name:
EIF2AK1 Rabbit Polyclonal Antibody
SKU:
CAB3415
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 371-630 of human EIF2AK1 (NP_055228.2).
The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
27102
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using [KO Validated] EIF2AK1 Rabbit pAb (CAB3415) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
