The EIF2A Polyclonal Antibody (CAB16205) is a valuable tool for researchers studying EIF2A, a protein involved in the regulation of protein synthesis in response to cellular stress. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.EIF2A plays a crucial role in the integrated stress response, which helps cells cope with various stressors such as nutrient deprivation, viral infection, and oxidative stress.
Dysregulation of EIF2A signaling has been linked to a variety of human diseases, including cancer, neurodegenerative disorders, and metabolic diseases.By using the EIF2A Polyclonal Antibody, researchers can detect and analyze EIF2A expression levels in different cell types and conditions, providing valuable insights into its role in cellular stress response mechanisms. This antibody is an essential tool for studies in molecular biology, cancer research, and cell signaling pathways.
Product Name:
eIF2A Rabbit Polyclonal Antibody
SKU:
CAB16205
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF2A (NP_114414.2).
This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
83939
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using eIF2A antibody (CAB16205) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
