The EID3 Polyclonal Antibody (PACO19588) is a valuable tool for research involving EID3, a transcriptional regulator involved in various cellular processes, including cell proliferation and differentiation. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.EID3, also known as EP300-interacting inhibitor of differentiation 3, is implicated in the regulation of gene expression and is known to interact with the transcriptional co-activator EP300. Studies have shown that EID3 plays a role in cell fate determination and development, making it a key target for research in fields such as developmental biology and oncology.
The EID3 Polyclonal Antibody binds specifically to the EID3 protein, allowing for accurate detection and analysis in a variety of cell types. Its high specificity and sensitivity make it an ideal tool for investigating the function of EID3 in different cellular processes and disease states. Researchers studying gene regulation, cell differentiation, and tumorigenesis will find this antibody invaluable for their experiments.
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using PACO19588(EID3 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: x200).
Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse kidney tissue, Primary antibody: PACO19588(EID3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO19588(EID3 Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
EID3 is highly expressed in testis and shows homology to a region of EID1 implicated in binding to CBP/p300. EID3 acts as a potent inhibitor of nuclear receptor transcriptional activity by a mechanism that is independent of direct interactions with nuclear receptors, including SHP. Furthermore, EID3 directly binds to and blocks the SRC-1 interacting domain of CBP, which has been implicated to act as the interaction surface for nuclear receptor co-activators. Consistent with this idea, EID3 prevents recruitment of CBP to a natural nuclear receptor-regulated promoter.
Synonyms:
EP300 interacting inhibitor of differentiation 3
UniProt Protein Function:
EID3: Tissue-specific component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components. Belongs to the NSE4 family.Chromosomal Location of Human Ortholog: 12q23.3Cellular Component: chromosome, telomeric region; cytoplasm; nucleusMolecular Function: identical protein binding; protein bindingBiological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; DNA repair; DNA recombination
E1A-like inhibitor of differentiation 3; EID-1-like inhibitor of differentiation 3; Non-structural maintenance of chromosomes element 4 homolog B; NS4EB; Non-SMC element 4 homolog B
