The EHMT2 Polyclonal Antibody (CAB1247) is a valuable tool for researchers studying EHMT2, also known as G9a, a histone methyltransferase involved in epigenetic regulation. This antibody, generated in rabbits, has high specificity for EHMT2 in human samples and has been validated for use in Western blot applications.EHMT2 plays a critical role in gene regulation by catalyzing the methylation of histone H3 at lysine 9, a modification associated with transcriptional repression. Dysregulation of EHMT2 has been linked to various diseases, including cancer and neurological disorders, making it a promising target for therapeutic interventions.
By using the EHMT2 Polyclonal Antibody, researchers can detect and analyze the expression levels of EHMT2 in different cell types and tissues, providing valuable insights into its function and potential implications in disease development. This antibody is a valuable tool for studies in epigenetics, cancer biology, and drug discovery, allowing researchers to further explore the role of EHMT2 in health and disease.
Product Name:
[KO Validated] EHMT2 Rabbit Polyclonal Antibody
SKU:
CAB1247
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 877-1176 of human EHMT2 (NP_079532.5).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
G9A; BAT8; GAT8; NG36; KMT1C; C6orf30; T2
Positive Sample:
U-937,293T,Jurkat,MCF7,HepG2
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
132kDa
Observed MW:
150kDa/170kDa
This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
10919
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] EHMT2 Rabbit pAb (CAB1247) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
