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[KO Validated] EHMT2 Rabbit Polyclonal Antibody (CAB1247)

SKU:
CAB1247
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • KO Validated Antibodies 1 Anti-EHMT2 Antibody CAB1247KO Validated
  • KO Validated Antibodies 1 Anti-EHMT2 Antibody CAB1247KO Validated
€139 - €419
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Description

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Product Name:[KO Validated] EHMT2 Rabbit Polyclonal Antibody
SKU:CAB1247
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 877-1176 of human EHMT2 (NP_079532.5).
Sequence:VGNR AIRT EKII CRDV ARGY ENVP IPCV NGVD GEPC PEDY KYIS ENCE TSTM NIDR NITH LQHC TCVD DCSS SNCL CGQL SIRC WYDK DGRL LQEF NKIE PPLI FECN QACS CWRN CKNR VVQS GIKV RLQL YRTA KMGW GVRA LQTI PQGT FICE YVGE LISD AEAD VRED DSYL FDLD NKDG EVYC IDAR YYGN ISRF INHL CDPN IIPV RVFM LHQD LRFP RIAF FSSR DIRT GEEL GFDY GDRF WDIK SKYF TCQC GSEK CKHS AEAI ALEQ SRLA RLDP HPEL LPEL GSLP PVNT
Tested Applications:WB IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:G9A; BAT8; GAT8; NG36; KMT1C; C6orf30; T2
Positive Sample:U-937,293T,Jurkat,MCF7,HepG2
Conjugate:Unconjugated
Cellular Localization:Chromosome, Nucleus.
Calculated MW:132kDa
Observed MW:150kDa/170kDa

This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:10919
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] EHMT2 Rabbit pAb (CAB1247) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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