The EHHADH Polyclonal Antibody (CAB5717) is a versatile tool for researchers studying the EHHADH protein, which plays a key role in fatty acid metabolism. This antibody, raised in rabbits, boasts high reactivity with human samples and is validated for use in Western blot applications.EHHADH, also known as enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase, is a crucial enzyme involved in the breakdown of fatty acids for energy production. Dysregulation of EHHADH has been linked to metabolic disorders and diseases such as obesity, diabetes, and cardiovascular conditions. By using the EHHADH Polyclonal Antibody, researchers can accurately detect and analyze EHHADH levels in various cell types, providing valuable insights into metabolic processes and potential therapeutic targets.
With its specificity and sensitivity, the EHHADH Polyclonal Antibody is a valuable tool for advancing research in the fields of biochemistry, metabolism, and metabolic diseases. Its ability to target the EHHADH protein in various experimental settings makes it an essential asset for scientists seeking to deepen their understanding of fatty acid metabolism and its implications for human health.
Product Name:
EHHADH Rabbit Polyclonal Antibody
SKU:
CAB5717
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 444-723 of human EHHADH (NP_001957.2).
SW480,A-549,HeLa,HepG2,Mouse small intestine,Rat liver,Rat kidney
Conjugate:
Unconjugated
Cellular Localization:
Peroxisome.
Calculated MW:
79kDa
Observed MW:
79kDa
The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1962
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EHHADH antibody (CAB5717) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
