The EHD3 Polyclonal Antibody (PAC047114) is a valuable tool for researchers studying EHD3, a key protein involved in intracellular membrane trafficking and recycling processes. This antibody, produced in rabbits, has high specificity for detecting EHD3 in human samples and is validated for use in Western blot applications. By targeting the EHD3 protein, researchers can accurately identify and study its function in various cell types, providing insight into cellular trafficking mechanisms and potential therapeutic targets.EHD3, a member of the EHD protein family, is known for its role in regulating endocytic pathways and maintaining cellular homeostasis.
Its involvement in membrane trafficking processes makes it a promising target for research on diseases related to cellular dysfunction, such as neurodegenerative disorders and metabolic syndromes. By understanding the function of EHD3, researchers can uncover new opportunities for developing treatments that target intracellular trafficking pathways and improve cellular function in disease states.
Immunohistochemistry of paraffin-embedded human brain tissue using PACO47114 at dilution of 1:100.
Immunofluorescent analysis of HepG2 cells using PACO47114 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO47114 at dilution of 1:100.
Background:
ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes tubulation of endocytic membranes. Binding to phosphatidic acid, induces its membrane tubulation activity (By similarity). Plays a role in endocytic transport. Involved in early endosome to recycling endosome compartment (ERC), retrograde early endosome to Golgi, and endosome to plasma membrane (rapid recycling) protein transport. Involved in the regulation of Golgi maintenance and morphology. Involved in the recycling of internalized D1 dopamine receptor. Plays a role in cardiac protein trafficking probably implicating ANK2. Involved in the ventricular membrane targeting of SLC8A1 and CACNA1C and probably the atrial membrane localization of CACNA1GG and CACNA1H implicated in the regulation of atrial myocyte exitability and cardiac conduction (By similarity). In conjunction with EHD4 may be involved in endocytic trafficking of KDR/VEGFR2 implicated in control of glomerular function (By similarity). Involved in the rapid recycling of integrin beta-3 implicated in cell adhesion maintenance. Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (By similarity). Plays a role in the formation of the ciliary vesicle, an early step in cilium biogenesis; possibly sharing redundant functions with EHD1.
Synonyms:
EH domain-containing protein 3 (PAST homolog 3), EHD3, EHD2 PAST3
UniProt Protein Function:
EHD3: Plays a role in endocytic transport.Chromosomal Location of Human Ortholog: 2p21Cellular Component: cytoplasm; endosome membrane; focal adhesion; nucleus; recycling endosome membraneMolecular Function: nucleic acid binding; protein bindingBiological Process: blood coagulation; cilium biogenesis; endocytic recycling; protein homooligomerization; receptor recycling
