The EGR1/EGR2 Polyclonal Antibody (PAC021743) is a valuable tool for researchers studying the early growth response (EGR) transcription factors EGR1 and EGR2. These transcription factors play key roles in the regulation of gene expression, cell proliferation, and differentiation. This antibody is raised in rabbits and is highly specific for detecting EGR1 and EGR2 proteins in human samples. It has been validated for use in Western blot applications, allowing for the detection and analysis of these transcription factors in various cell types.
Research involving EGR1 and EGR2 is important in understanding their role in processes such as cell growth, development, and response to environmental stimuli. Dysregulation of EGR1 and EGR2 has been implicated in various diseases, including cancer and neurological disorders. Therefore, this antibody is a valuable tool for researchers studying these transcription factors in immunology, cancer research, and other areas of biomedical research.
Antibody Name:
EGR1/EGR2 Antibody (PACO21743)
Antibody SKU:
PACO21743
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from internal of human EGR1/2.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Wright J.J., Science 248:588-591(1990).
Clonality:
Polyclonal
Conjugate:
Non-conjugated
Western blot analysis of extracts from HeLa/Jurkat cells, using EGR1/2 antibody.
Synonyms:
AT225; EGR-1; Early growth response protein 1; KROX-24; Krox-24 protein
UniProt Protein Function:
EGR1: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. By growth factors. Belongs to the EGR C2H2-type zinc-finger protein family.
UniProt Protein Details:
Protein type:DNA-binding; C2H2-type zinc finger protein; Transcription factor
Molecular Function:histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; metal ion binding; double-stranded DNA binding; transcription factor binding; transcription factor activity
Biological Process: regulation of long-term neuronal synaptic plasticity; circadian rhythm; response to nutrient levels; transcription from RNA polymerase II promoter; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; response to amphetamine; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; BMP signaling pathway; response to ethanol; learning and/or memory; cellular response to insulin stimulus; positive regulation of neuron apoptosis; response to glucose stimulus; oligodendrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; response to electrical stimulus; T cell differentiation; regulation of protein sumoylation; negative regulation of apoptosis
NCBI Summary:
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene. [provided by RefSeq, Dec 2014]
TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268Â Â
NCBI Protein Information:
early growth response protein 1; EGR-1; zinc finger protein 225; transcription factor ETR103; transcription factor Zif268; zinc finger protein Krox-24; nerve growth factor-induced protein A
UniProt Protein Name:
Early growth response protein 1
UniProt Synonym Protein Names:
AT225; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Zinc finger protein Krox-24
