The EGL Nine Homolog 1 Polyclonal Antibody (CAB10342) is a valuable tool for researchers studying EGLN1, a protein involved in the regulation of cellular response to hypoxia. This antibody, produced using rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By specifically binding to the EGLN1 protein, researchers can easily detect and analyze its expression in various cell types, making it an ideal tool for studies in hypoxia-related diseases and conditions.EGLN1, also known as HIF prolyl hydroxylase 2 (PHD2), plays a crucial role in the regulation of hypoxia-inducible factor (HIF) stability, which in turn regulates the cellular response to low oxygen levels.
Aberrant EGLN1 activity has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions. Understanding the function of EGLN1 is key to developing targeted therapies for these diseases, making the EGL Nine Homolog 1 Polyclonal Antibody an essential tool for researchers in the field.
Product Name:
EGLN1/EGLN2 Rabbit Polyclonal Antibody
SKU:
CAB10342
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human EGLN1/EGLN2 (NP_071334.1/NP_444274.1).
cytoplasm, cytosol, glutamatergic synapse, nucleus, postsynaptic density
Calculated MW:
36kDa/43kDa/46kDa/40kDa
Observed MW:
44kDa
The proteins encoded by EGLN1/EGLN2 gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex.
Purification Method:
Affinity purification
Gene ID:
54583/112398
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using EGLN1/EGLN2 antibody (CAB10342) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
