The EGLN1 Polyclonal Antibody (CAB2314) is designed for research involving EGLN1, a key enzyme involved in the regulation of cellular responses to hypoxia. The antibody, raised in rabbits, is highly specific and reactive with human samples, making it an ideal tool for studying the role of EGLN1 in various biological processes.EGLN1, also known as prolyl hydroxylase domain-containing protein 2 (PHD2), is a critical regulator of the hypoxia-inducible factor (HIF) pathway, which plays a central role in cellular adaptation to low oxygen levels.
Dysregulation of this pathway has been linked to a variety of diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions.By targeting EGLN1 with this antibody, researchers can analyze the expression and activity of the enzyme in different cell types and tissues. This information is essential for understanding the molecular mechanisms underlying hypoxia-related diseases and developing targeted therapies to modulate EGLN1 activity for therapeutic purposes.
Product Name:
PHD2 Rabbit Polyclonal Antibody
SKU:
CAB2314
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-426 of human PHD2/EGLN1 (NP_071334.1).
The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
Purification Method:
Affinity purification
Gene ID:
54583
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Immunofluorescence analysis of MCF-7 cells using PHD2/EGLN1 antibody (CAB2314). Blue: DAPI for nuclear staining.
