The EGLN1 Polyclonal Antibody (CAB14557) is a valuable tool for research involving the EGLN1 protein, which is involved in the regulation of cellular response to hypoxia. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the EGLN1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in the fields of hypoxia research and cancer biology.EGLN1, also known as PHD2, plays a critical role in regulating the stability of hypoxia-inducible factor (HIF) under conditions of low oxygen levels.
Dysregulation of EGLN1 has been implicated in a variety of diseases, including cancer, cardiovascular disorders, and neurological conditions. Understanding the function of EGLN1 is essential for developing targeted therapies that manipulate cellular responses to hypoxia in these disease contexts. Overall, the EGLN1 Polyclonal Antibody (CAB14557) is a reliable tool for researchers looking to investigate the role of EGLN1 in cellular hypoxic responses and its potential implications in various disease states.
Product Name:
[KO Validated] PHD2 Rabbit Polyclonal Antibody
SKU:
CAB14557
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PHD2/EGLN1 (NP_071334.1).
Sequence:
MAND SGGP GGPS PSER DRQY CELC GKME NLLR CSRC RSSF YCCK EHQR QDWK KHKL VCQG SEGA LGHG VGPH QHSG PAPP AAVP PPRA GARE PRKA AARR
The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
Purification Method:
Affinity purification
Gene ID:
54583
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and PHD2/EGLN1 knockout (KO) 293T cells, using [KO Validated] PHD2/EGLN1 Rabbit pAb (CAB14557) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
