The EGL-9 Homolog 3 Polyclonal Antibody (CAB8001) is a valuable tool for researchers studying EGL-9 homolog 3, a protein involved in cellular oxygen sensing and regulation of hypoxia-inducible factor (HIF) activity. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.EGL-9 homolog 3 is a key player in the cellular response to changes in oxygen levels, making it crucial for understanding processes like angiogenesis, metabolism, and cell survival under hypoxic conditions. By targeting EGL-9 homolog 3 with this antibody, researchers can detect and analyze the protein in various cell types, providing insights into its role in oxygen sensing and HIF regulation.
With its ability to specifically bind to EGL-9 homolog 3, this polyclonal antibody is an essential tool for studies in the fields of cellular biology, metabolism, and cancer research. By investigating the mechanisms through which EGL-9 homolog 3 influences cellular responses to hypoxia, researchers can uncover new therapeutic targets for diseases associated with oxygen imbalance, such as cancer and cardiovascular disorders.
Product Name:
PHD3 Rabbit Polyclonal Antibody
SKU:
CAB8001
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PHD3 (NP_071356.1).
Enables peptidyl-proline 4-dioxygenase activity. Involved in several processes, including activation of cysteine-type endopeptidase activity involved in apoptotic process; peptidyl-proline hydroxylation to 4-hydroxy-L-proline; and response to hypoxia. Located in cytosol and nucleus. Implicated in renal cell carcinoma. Biomarker of clear cell renal cell carcinoma.
Purification Method:
Affinity purification
Gene ID:
112399
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using PHD3 Rabbit pAb (CAB8001) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
