The EGL Nine Homolog 2 Polyclonal Antibody (CAB2252) is a valuable tool for researchers studying EGL Nine Homolog 2, a protein involved in various cellular processes, including cell cycle regulation and tumorigenesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.EGL Nine Homolog 2, also known as EglN2 or PHD1, is a member of the prolyl hydroxylase family and plays a key role in oxygen sensing and response. Dysregulation of EGL Nine Homolog 2 has been implicated in various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions.
By targeting EGL Nine Homolog 2 with this polyclonal antibody, researchers can detect and analyze the protein in different cell types, shedding light on its function and potential therapeutic implications. This antibody is a valuable tool for studies in cancer biology, hypoxia response, and cellular metabolism, offering new insights into the role of EGL Nine Homolog 2 in health and disease.
The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
Purification Method:
Affinity purification
Gene ID:
112398
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Prolyl hydroxylase PHD1 (EGLN2) Rabbit pAb (CAB2252) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
