The EGFR Polyclonal Antibody (CAB21740) is a valuable tool for researchers studying the epidermal growth factor receptor (EGFR), a critical signaling molecule involved in cell growth and differentiation. This antibody, produced in rabbits, specifically targets EGFR and is highly reactive with human samples, making it an excellent choice for Western blot applications.EGFR is a transmembrane protein that plays a key role in regulating cell proliferation, survival, and migration. Dysregulation of EGFR signaling has been linked to various cancers, making it a promising target for cancer therapy.
The EGFR Polyclonal Antibody enables the detection and analysis of EGFR expression in different cell types, offering valuable insights into its role in cancer progression and other diseases.By studying EGFR with this antibody, researchers can gain a better understanding of its signaling pathways and potential therapeutic interventions for EGFR-related diseases. Its reliability and specificity make it an essential tool for immunology and cancer research.
Product Name:
EGFR Polyclonal Antibody
SKU:
CAB21740
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human EGFR (NP_005219.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa/
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and EGFR knockdown (KD) HeLa cells using [KD Validated] EGFR Rabbit pAb (CAB21740) at 1:5000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020)Exposure time: 0.5 s.
