The EGFR Polyclonal Antibody (CAB2069) is a valuable tool for research involving the epidermal growth factor receptor (EGFR), a critical player in cell growth, proliferation, and differentiation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. It specifically binds to the EGFR protein, enabling accurate detection and analysis in a variety of cell types, making it ideal for studies in cancer biology and signal transduction pathways.
EGFR is a transmembrane receptor that is overexpressed in many solid tumors, making it a promising therapeutic target for cancer treatment. The antibody provides researchers with a tool to investigate EGFR signaling pathways, its role in tumorigenesis, and potential therapeutic interventions targeting this receptor. Understanding the intricacies of EGFR function is essential for the development of new cancer therapies that specifically target this receptor and improve patient outcomes.
Product Name:
[KO Validated] EGFR Rabbit Polyclonal Antibody
SKU:
CAB2069
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1100-1200 of human EGFR (NP_005219.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa//175kDa/175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using EGFR Rabbit pAb (CAB2069) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
