The EGFR Polyclonal Antibody (CAB11577) is specifically designed for research involving the Epidermal Growth Factor Receptor (EGFR), a key cell surface receptor involved in promoting cell growth and survival. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the EGFR protein, this antibody allows for the detection and analysis of EGFR expression in various cell types, making it an essential tool for studies in cancer research and molecular biology. EGFR is known for its role in regulating cell proliferation, survival, and differentiation, and aberrant EGFR signaling has been implicated in various cancers, including lung, colorectal, and breast cancers.
Understanding the role of EGFR in cancer initiation and progression is of paramount importance for developing targeted therapies and personalized treatment strategies. The EGFR Polyclonal Antibody (CAB11577) offers researchers a reliable tool to study EGFR expression and signaling pathways, thereby contributing to advancements in cancer therapeutics and precision medicine.
Product Name:
[KO Validated] EGFR Rabbit Polyclonal Antibody
SKU:
CAB11577
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and EGFR knockdown (KD) HeLa cells, using [KD Validated] EGFR Rabbit pAb (CAB11577) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.5s.
