The EGFR Monoclonal Antibody (CAB23452) is a valuable tool for researchers studying the Epidermal Growth Factor Receptor (EGFR) pathway, a key signaling pathway involved in cell growth, proliferation, and differentiation. This antibody, developed through monoclonal antibody technology, specifically targets the EGFR protein, allowing for precise detection and analysis in a variety of experimental settings.EGFR is a transmembrane receptor tyrosine kinase that plays a crucial role in various cellular processes, making it a significant target for cancer research, particularly in the development of targeted therapies for cancer treatment.
This antibody is highly specific for human samples and has been validated for use in techniques such as Western blotting, immunoprecipitation, and immunofluorescence.By utilizing the EGFR Monoclonal Antibody (CAB23452), researchers can gain valuable insights into the role of EGFR in cancer biology, drug resistance mechanisms, and potential therapeutic strategies targeting the EGFR pathway. Its high specificity and sensitivity make it an essential tool for studies focused on understanding and manipulating EGFR signaling in both normal and pathological conditions.
Product Name:
EGFR Monoclonal Antibody
SKU:
CAB23452
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant protein of human EGFR.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; FR
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Clone Number:
ARC56230
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and EGFR knockdown (KD) HeLa cells, using [KD Validated] EGFR Rabbit mAb (CAB23452) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
