The EGFR L858R Rabbit Monoclonal Antibody (CAB5031) is a specialized antibody designed for research involving the EGFR L858R mutation, a common mutation found in non-small cell lung cancer. This antibody, generated in rabbits, is highly specific and sensitive for detecting the EGFR L858R mutation in human samples using techniques such as immunohistochemistry and Western blot.The EGFR L858R mutation is known to drive tumorigenesis and cancer progression in non-small cell lung cancer, making it a critical target for therapeutic intervention. By using the EGFR L858R Rabbit Monoclonal Antibody, researchers can accurately detect and analyze the presence of this mutation in cancer samples, aiding in the development of targeted therapies and personalized treatment strategies for patients with EGFR L858R-mutated tumors.
The high specificity and sensitivity of this antibody make it a valuable tool for studying the role of the EGFR L858R mutation in cancer biology and for identifying potential biomarkers for patient stratification and prognosis. Its use in research applications can provide valuable insights into the molecular mechanisms underlying EGFR mutation-driven tumorigenesis and aid in the development of novel targeted therapies for non-small cell lung cancer.
Product Name:
EGFR (L858R) Rabbit Monoclonal Antibody
SKU:
CAB5031
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 800-900 of human EGFR (L858R) (P00533).
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Clone Number:
ARC1139
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brain , using EGFR (L858R) Rabbit mAb (CAB5031) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
