The EGFR Polyclonal Antibody (CAB11082) is a valuable tool for researchers studying the epidermal growth factor receptor (EGFR), a cell surface protein involved in cell growth and survival pathways. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.EGFR is a key player in various cellular processes, including cell proliferation, differentiation, and migration. Dysregulation of EGFR signaling has been implicated in numerous diseases, including cancer, making it an attractive target for therapeutic intervention.
By using the EGFR Polyclonal Antibody, researchers can detect and analyze EGFR expression in different cell types, providing valuable insights into its role in cancer biology and other disease processes. This antibody is a valuable tool for furthering our understanding of EGFR biology and developing targeted therapies for EGFR-associated diseases.
Product Name:
EGFR Rabbit Polyclonal Antibody
SKU:
CAB11082
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1026-1126 of human EGFR (NP_005219.2).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cellsIF/ICC,1:50 - 1:200
Synonyms:
ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; FR
Positive Sample:
HeLa,Mouse liver,Rat liver,HeLa
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein.
Calculated MW:
134kDa
Observed MW:
175kDa/
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Purification Method:
Affinity purification
Gene ID:
1956
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse liver, using [KD Validated] EGFR Rabbit pAb (CAB11082) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
